Welcome back to another Free Stuff Friday… well technically it's Saturday but who's counting right. Wooooooh what a busy week. All kinds of exciting things happening at Bob Ostrom Studio. More eBooks, more eBooks. Don't worry people I hear you shouting I have lots more eBooks on the way, and since you read my blog you'll be the first ones to know when they hit the market.
January 28, 2012 Comments Off
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It's no secret to lower mainlander's that housing prices are sky high. The recent Demographia survey indicates that Vancouver is now the 2nd costliest housing market in the world, just behind Hong Kong and ahead of Sydney. The city has become so unaffordable for most that many are forced to the far flung suburbs to toil away in rush hour traffic day in and day out. The amount of million dollar detached homes is ridiculous, just check out this map of million dollar homes in Vancouver.
Here is a blurb from Bloomberg Businesweek:
Vancouver displaced Sydney as the least-affordable housing market after Hong Kong among large English-speaking cities, as home prices rose faster than incomes, a study of 325 metropolitan areas worldwide showed.
So, in an England of the future where the right to vote is dictated by the willingness to carry a knife and engage in knife duels and challenges, a young man runs away from his psychiatrist's office and the young man's father goes on a legal rampage against the psychiatrist. He also hires our knife wielding hero to find his son.
The prime minister's flying visit to the Council of Europe's Parliamentary Assembly today is mission undeliverable, even if many may feel Cameron has a good case. But the way he has pandered to the worst atavistic elements of the Europhobe right and the clamour of the offshore press for retributionist punishment of prisoners means he will hardly get a hearing.
A simple solution to the issue of prisoners' voting rights, for example, would be to do what the French do, which is to empower judges to add an additional sentence of loss of civic rights for those imprisoned for serious crimes. This is in conformity with ECHR judgements. Switzerland, the dream nation for anti-EU Tories, has allowed its prisoners to vote for 40 years as have all the more civilised European nations.
Copyright 2011 Yeon Suk Jung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of dendritic cells (DCs). DCs are specialized antigen-presenting cells, and they interact with T cells and B cells to initiate immune responses. Phenotypic changes in DCs, such as the production of CD86 and CD54 and internalization of MHC class II molecules, have become focal points of the skin sensitization test. In this study, we used h-CLAT to assess the effects of biodegradable polymers. The results showed that several biodegradable polymers increased the expression of CD54, and the relative skin sensitizing abilities of biodegradable polymers were PLLG (75 : 25) < PLLC (40 : 60) < PLGA (50 : 50) < PCG (50 : 50). These results may contribute to the creation of new guidelines for the use of biodegradable polymers in scaffolds or allergenic hazards.
Until recently, studies of skin sensitization have used the guinea pig maximization test (GPMT) [1–3]. Currently, the local lymph node assay (LLNA) [4, 5] is often used as an alternative to GPMT. The advantages of LLNA are the ability to make dose-dependent evaluations, a decrease in animal usage, shorter experimental time periods, and lower costs [6, 7]. In addition, a movement to prohibit the use of animals for safety testing of new materials is spreading throughout the world [8]. Recently, Ashikage et al. (2006) [9], Sakaguchi et al. (2006) [10] and several laboratories have reported the use of a new alternative to animal experiments, the human cell line activation test (h-CLAT) [11, 12].
Dendritic cells (DCs) are in contact with the skin and inner lining of the nose, lungs, stomach, and intestines [13]. They can also be found in an immature state in the blood. Antigens from viruses and microbes not only induce an adaptive immune response in DCs but also induce innate immunity to activate the immune system [14–16]. When skin sensitization develops, DCs immigrate to the secondary lymphoid organs to present naïve T cells [17]. Then, immature DCs mature and intercellular adhesion molecules, costimulatory molecules, and major histocompatibility complex II (MHC II) antigens (CD54, CD86, HLA-DR antigens) [18–21]. The immune reaction of DCs remains weak without costimulatory molecules. Therefore, skin sensitization in in vitro experiments is judged by expressions of CD54 and CD86.
THP-1 cells, a human monocyte cell line, are used in place of DCs in h-CLAT. We can evaluate phenotypical alterations on these THP-1 cells. In the present studies, we assessed skin sensitization to biodegradable polymers by in vitro measures.
THP-1 cells purchased from American Type Culture Collection (Manassas, Va, USA) were cultured with RPMI Medium 1640 (GIBCO, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Intergen, Purchase, NY, USA), 0.05 mM 2-mercaptoethanol (GIBCO), and 1% streptomycin (GIBCO). The cells used in testing were between 2 weeks to 2 months old.
Biodegradable polymers P1 to P6 (Table 1) were obtained from Taki Chemical Co., Ltd. (Hyogo, Japan). We made 6 initial stock solutions by dissolving the biodegradable polymers in dimethyl sulfoxide (DMSO, Sigma, Ayrshire, UK). The biodegradable polymers P1, P2, P3, P4, P5, and P6 were dissolved separately in DMSO. 2,4-Dinitrochlorobenzene (DNCB, Sigma-Aldrich, St. Louis, Mo, USA) was also dissolved in DMSO and used as a positive control for the skin sensitization test. We made 6 substock solutions of each polymer using two-times dilution series. These sub-stock solutions were diluted 20 times with RPMI medium 1640. Finally, the working solutions were added to the cells at 100 times dilution.
Table 1: Condition and composition of biodegradable polymers.
September 5, 2011
Java game, gundam, java, split, splitting, sprite, sprites
In this tutorial I will show you how easy to draw (character) sprite if you wanna make game in java programing language. First we gonna use JPanel as our game canvas, the idea is override and call logic method as you need inside of it, example :
How Do You Calculate the Amount of Watts per Gallon, I Am Doing the Research and Buying a T5 Light Fixture This Weekend Not Sure Which One...
Now lighting efficiency is a different story. The amount of useable light directed towards a subject is more what you are interested in.
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I went completely out of comfort zone today and drove to PhillippePark in Safety Harbor. It was a treat. The majority of the park is on the water. It has picnic shelters, a great playground, and the largest native american mound in the Tampa Bay area. Bella had a blast playing on the playground that had multiplebenches and viewing points for parents. Matt and I enjoyed the climbing the mound and learning the history of the Tocobaga Indians.
From the press release:
Olathe, Kansas- #1 distributor OMB Guns announces SHOT Show teaming up with world champion competitive pistol shooter Dave Sevigny to promote the FNH USA new striker fired pistols to law enforcement….."SHIFT CHANGE" is here and proudly made in the USA!
President of OMB Guns John S. Ralph III stated, "Dave is a humble, polite champion that comes along once every 20 years. He demands high performance standards and already has provided us with valuable feedback to share with our manufacturers. At OMB Guns we value the synergy of world class shooting, dedicated work ethic and powerful sales experience to be the best."
